Coding

Part:BBa_K2110016:Design

Designed by: Le He   Group: iGEM16_Tianjin   (2016-10-06)


The key gene for utilization of ethylene glycolin P.putida KT2440.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 271
    Illegal NgoMIV site found at 436
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 823
    Illegal NgoMIV site found at 1081
    Illegal AgeI site found at 1294
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1309


Design Notes

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Source

P.putida KT2440 genome

References